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Nucleic Acids Research, 1989, Vol. 17, No. 10 3783-3793
© 1989

CHEMISTRY

Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques

B.A. Lasker*, G.F. Carle+, G.S. Kobayashi and G. Medoff

Divisions of Infectious Diseases, of Dermatology, and of Laboratory Medicine, Department of Medicine and Department of Genetics Washington University School of Medicine, St Louis, MO 63110, USA

Received November 16, 1988. Revised April 12, 1989. Accepted April 12, 1989.

Pulsed-field gel electrophoresis techniques were used to study chromosome-sized DNA molecules of C. albicans. Chromosome-sized DNA of two strains of Candida albicans has been resolved into 8 bands by orthogonal-field-alternation gel electrophoresis (OFAGE). Six bands were observed in chromosomal preparations of C. albicans using field-inversion gel electrophoresis (FIGE). Differences in the electrophoretic mobilities of bands of the strains of C. albicans examined suggests that chromosome-length polymorphisms exist and make it difficult to correlate the banding patterns among strains. These correlations were facilitated, however, by assignment of C. albicans chromosomes by hybridization using a collection of cloned DNA probes specific for each of the 8 observed bands. Southern blotting showed that the 6 FIGE bands consisted of 4 singlets and 2 comigrating doublets, accounting for the 8 bands observed by OFAGE analysis. The agreement between OFAGE and FIGE analysis suggests that the C. albicans haploid genome contains a minimum of 8 chromosomes.

*Present addresses BAL. Mycology Section. Center for Disease Control. Atlanta, GA 30333. USA

+Present addresses GFC, Centre de Biochimie. Université de Nice. 06034 Nice Cedex. France


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