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Nucleic Acids Research, 2000, Vol. 28, No. 8 E33-00
© 2000 Oxford University Press

Oligonucleotide immobilization on micropatterned streptavidin surfaces

Chandran R. Sabanayagam1,2,*, Cassandra L. Smith1,2,3 and Charles R. Cantor1,3,4

1Center for Advanced Biotechnology, 2Department of Biology, 3Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA and 4Sequenom, Inc., 11555 Sorrento Valley Road, San Diego, CA 92121, USA

We describe a simple procedure for photolithographic patterning of streptavidin on silicon substrates. Long wavelength UV (365 nm) light was used to direct the covalent attachment of photoactivatable biotin onto silylated silicon wafers. Fluorescently labeled streptavidin was found to bind only in areas exposed to the light. We used this procedure to selectively pattern streptavidin inside microwells etched in silicon, and we investigated the binding characteristics of biotinylated oligonucleotides of lengths, n = 16, 54 and 99 bases. The binding curves were found to fit the functional form of the Langmuir isotherm, with binding saturation proportional to n–3/4.

* To whom correspondence should be addressed at: Center for Advanced Biotechnology, Boston University, 36 Cummington Street, 2nd Floor, Boston, MA 02215, USA. Tel: +1 617 353 8505; Fax: +1 617 353 8501; Email: chandran@bu.edu


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[Abstract] [Full Text] [PDF]


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