Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (55K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Watanabe, J.
Right arrow Articles by Sugano, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Watanabe, J.
Right arrow Articles by Sugano, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 1 70-71
© 2001 Oxford University Press

FULL-malaria: a database for a full-length enriched cDNA library from human malaria parasite, Plasmodium falciparum

Junichi Watanabe*, Masahide Sasaki1, Yutaka Suzuki1 and Sumio Sugano1

Department of Parasitology and 1Department of Virology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo 108-8639, Japan

FULL-malaria is a database for a full-length-enriched cDNA library from the human malaria parasite Plasmodium falciparum (http://133.11.149.55/). Because of its medical importance, this organism is the first target for genome sequencing of a eukaryotic pathogen; the sequences of two of its 14 chromosomes have already been determined. However, for the full exploitation of this rapidly accumulating information, correct identification of the genes and study of their expression are essential. Using the oligo-capping method, we have produced a full-length-enriched cDNA library from erythrocytic stage parasites and performed one-pass reading. The database consists of nucleotide sequences of 2490 random clones that include 390 (16%) known malaria genes according to BLASTN analysis of the nr-nt database in GenBank; these represent 98 genes, and the clones for 48 of these genes contain the complete protein-coding sequence (49%). On the other hand, comparisons with the complete chromosome 2 sequence revealed that 35 of 210 predicted genes are expressed, and in addition led to detection of three new gene candidates that were not previously known. In total, 19 of these 38 clones (50%) were full-length. From these obser­vations, it is expected that the database contains ~1000 genes, including 500 full-length clones. It should be an invaluable resource for the development of vaccines and novel drugs.

* To whom correspondence should be addressed. Tel: +81 3 3443 8111; Fax: +81 3 5449 5402; Email: jwatanab{at}manage.ims.u-tokyo.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Watanabe, H. Wakaguri, M. Sasaki, Y. Suzuki, and S. Sugano
Comparasite: a database for comparative study of transcriptomes of parasites defined by full-length cDNAs
Nucleic Acids Res., January 12, 2007; 35(suppl_1): D431 - D438.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Balu, D. A. Shoue, M. J. Fraser Jr., and J. H. Adams
High-efficiency transformation of Plasmodium falciparum by the lepidopteran transposable element piggyBac
PNAS, November 8, 2005; 102(45): 16391 - 16396.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Watanabe, Y. Suzuki, M. Sasaki, and S. Sugano
Full-malaria 2004: an enlarged database for comparative studies of full-length cDNAs of malaria parasites, Plasmodium species
Nucleic Acids Res., January 1, 2004; 32(90001): D334 - 338.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.