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Nucleic Acids Research, 2002, Vol. 30, No. 1 98-102
© 2002 Oxford University Press

RiceGAAS: an automated annotation system and database for rice genome sequence

Katsumi Sakata*, Yoshiaki Nagamura, Hisataka Numa, Baltazar A. Antonio1, Hideki Nagasaki1, Atsuko Idonuma1, Wakako Watanabe2, Yuji Shimizu2, Ikuo Horiuchi2, Takashi Matsumoto, Takuji Sasaki and Kenichi Higo

National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan, 1Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan and 2Mitsubishi Space Software Co. Ltd, 1-17-15 Sengen, Tsukuba, Ibaraki 305-0047, Japan

An extensive effort of the International Rice Genome Sequencing Project (IRGSP) has resulted in rapid accumulation of genome sequence, and >137 Mb has already been made available to the public domain as of August 2001. This requires a high-throughput annotation scheme to extract biologically useful and timely information from the sequence data on a regular basis. A new automated annotation system and database called Rice Genome Automated Annotation System (RiceGAAS) has been developed to execute a reliable and up-to-date analysis of the genome sequence as well as to store and retrieve the results of annotation. The system has the following functional features: (i) collection of rice genome sequences from GenBank; (ii) execution of gene prediction and homology search programs; (iii) integration of results from various analyses and automatic interpretation of coding regions; (iv) re-execution of analysis, integration and automatic interpretation with the latest entries in reference databases; (v) integrated visualization of the stored data using web-based graphical view. RiceGAAS also has a data submission mechanism that allows public users to perform fully automated annotation of their own sequences. The system can be accessed at http://RiceGAAS.dna.affrc.go.jp/.

* To whom correspondence should be addressed. Tel: +81 298 38 2199; Fax: +81 298 38 2302; Email: ksakata{at}nias.affrc.go.jp


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